Thesis Title: In vivo analysis of microtubulesevering proteins in motile cilia assembly
• Established a first connection between microtubule dynamics and cilia assembly
• Documented novel ultrastructural phenotypes by using confocal microscopy and electron
• Executed gene expression and knockout studies to analyzed the function of ciliary
• Discovered functional link between katanin and tubulin posttranslational modifications
• Achieved recognition by "faculty of " recommended and highly cited cover page article in
"The Journal of Cell Biology"
• Mammalian Cell Culture: Transfection, Lentivirus mediated infection, stable cell line
development, clone selection, immunocytochemistry
• Molecular Biology: Plasmid vector construction using regular and gateway cloning,
Recombinant DNA technology, gene manipulation and gene expression, PCR, RTPCR,
• Biochemistry: Protein expression and purification GST and Histagged, Subcellular
fractionation, SDSPAGE, Western blotting
• Microscopy: Confocal Scanning and Spinning Disk, Transmission, Immunoelectron and
Scanning electron microscopy, Image analysis Metamorph, Volocity
• Computer proficiency: PC/MAC, Adobe Photoshop, MSOffice Excel, Word, PowerPoint
Endnote, GraphPad Prism, Vector NTI, Sequence alignment
• S. N, et al. Soluble levels of cytosolic tubulin regulate ciliary length control. Molecular
Biology of the Cell. Jan .
• Neeraj S. and Bradley K. Yoder. Loss of primary cilium affects microtubule acetylation.
• Nicolas F. Berbari, Nicolas W. Kin, Neeraj S., Ed Michaud, John F. Kearney and
Bradley K. Yoder. Trafipmutant mice display phenotypes of primary cilia
• Dragos Olteanu, Xiaofen Liu, Wen Liu, Venus Roper, Neeraj S., Bradley Yoder, Lisa
Satlin, Erik Schwiebert, and Mark Bevensee. Increased Na+H+ Exchanger Activity on the
Apical Surface of a Ciliumdeficient Cortical Collecting Duct Principal Cell Model of Polycystic
Kidney Disease. Submitted
• Nathan E. Hellman, Yan Liu, David R. Beier, Christopher Ward, Zhaoxia Sun, Neeraj
S., Bradley K. Yoder, and Iain A. Drummond. foxja regulates cilia function in response
to injury and cystic kidney disease. Proc Natl Acad Sci U S A. Oct ;:
• Neeraj S., et al. Role of primary cilia, proliferation and kidney injury in development of
polycystic kidney disease. Manuscript in preparation.
• Drashti Dave, Neeraj S., Dorota Wloga and Jacek Gaertig. DYF is required for
assembly of the axoneme in Tetrahymena. Eukaryotic Cell. Sep;:.