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<pre>Array ( [var] => cut_url ) </pre> Resume for Mark J. for Educator / Teacher / Education & Science in Auburndale, Massachusetts. Search More Resumes for Educator / Teacher on Resumark.com #ICBA7A3MZ
 

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Resume for Mark J. for Educator / Teacher / Education & Science in Auburndale, Massachusetts




Occupation: Educator / Teacher Industry: Education & Science
Country: United States City: Auburndale
State: Massachusetts ZIP: 02466



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MARK E. J. Webster Street Auburndale, MA Cell: Home: Accomplished Molecular/Cell Biologist and Science Educator · · · · · More than years research experience in biochemistry, gene expression, and bioinformatics. Effective leader and team player with broad background in scientific processes and business approaches in academic, corporate, and nonprofit environments. Expertise in establishing up to date and costeffective processes in cell and molecular biology. Coauthor on peerreviewed scientific publications. Experienced inquirybased science educator skilled at making science and math relevant to students. AREAS OF SIGNIFICANT ACCOMPLISHMENT: SCIENCE EDUCATION · · · · · · MOLECULAR and CELL BIOLOGY · TRANSFECTION and PROTEIN EXPRESSION · · · BIOINFORMATICS LABORATORY MANAGEMENT · · · Teach biotechnology skills and concepts to high school students. Design original, stateoftheart biotechnology curriculum modules. Designed integrated inquiry chemistry, astronomy, and evolutionary biology curricula. Prepared and taught ecology and environmental education programs to a wide audience including schoolage children, and adults. Lead handson, activities instructing children on fundamentals of scientific principles. Certified in Project WILD and Project WILD Aquatic K curricula Demonstrated expertise in molecular cloning technologies including: Genomic and Plasmid cloning, RTPCR, Microarray analysis, Adenovirus expression, Oligodirected mutagenesis, dideoxyDNA sequencing, DNA/Protein sequence analysis, RNA isolation, RNAi, in vitro transcription, Southern, Northern, Western blot, S analysis, Implemented cellbased assay systems using a wide variety of plasmid constructs, transient and stable transfected cell types, and reporter systems. Established Standard Operating Procedures for transient and stable transfection of mammalian and lepidopteron cell lines. Organized and systematized tissue culture facilities, establishing parameters for accurate calibration of incubators, laminar flow hoods, and light/fluorescent microscopes. Validated SDSPAGE for quantification of expression levels using fluorescent staining methods. Solved IPrelated issue relevant to product marketing by using structural modeling and HMM Bioinformatics strategies. Designed and implemented DNA/protein sequence analyses using BLAST, ClustalW, PDB, DAMBE, SAGE, SwissModel, Pfam, VectorNTI, DNAstar, and other bioinformatics suites. Generated evolutionary sequencebased dendrograms, complete with bootstrapping, for development of potential drug targets. Created a resultsbased bioinformatics platform by incorporating complex inhouse software. Setup new postrelocation molecular biology and tissue culture facilities, establishing full functionality within hours. Established and managed all aspects of lab function, including safety protocols, purchasing, stocking, and tracking of reagents and supplies. Systematized multiple DNA expression plasmid constructs along with corresponding sequencing/mutagenic oligonucleotides using FileMaker Pro. PROFESSIONAL EXPERIENCE: CityLab at Boston University Medical Center, Boston, MA Present Science Educator Teach dynamic classes in biotechnology skills and applications to high school students. Also develop science curriculum based on stateoftheart chemistry and molecular biology technologies. Massachusetts Audubon Society, Belmont, MA Teacher/Naturalist Plan, prepare, and lead handson environmental science education programs instructing children and adults on fundamental scientific principles. Public School District, Brookline, MA Science Teacher Filled midyear staffing vacancy in a public school by taking responsibility for classes in eighth grade science, when teacher went out on leave. Developed curriculum, and taught Chemistry and Astronomy classes. Momenta Pharmaceuticals, Inc. Cambridge, MA Senior Research Associate Designed molecular biology, cell biology, and tissue culture protocols for this startup biotechnology company. Performed researched in glycomic molecular/cell biology in the Recombinant Enzyme and Oncology Teams. Millennium Pharmaceuticals, Inc. Cambridge, MA Senior Research Associate Conducted gene mining and discovery for Ion Channel, Adenovirus, Apoptosis, and Obesity Programs. Designed molecular and cell biology protocols in Neurobiology and Metabolic Disease Biology Departments. Developed a resultsbased bioinformatics platform. Laboratory of Gary I. Yellen, Baltimore, MD and Boston, MA Johns Hopkins University and Massachusetts General Hospital Laboratory Manager and Research Technician Systematized all molecular and cell biology protocols in active research laboratory, relying on these techniques for physiological analysis of membrane ion channels. Managed all aspects of lab function. Laboratory of Joseph D. Mosca, Baltimore, MD Johns Hopkins University, Dept. of Oncology Research Technician Performed molecular biology and cell culture in laboratory characterizing the regulation and expression of DNA from the Human Immunodeficiency Virus HIV. EDUCATION: Lesley University, Cambridge, MA Master of Science ­ Middle School Science Education Northeastern University, Boston MA Graduate Certificate in Bioinformatics Boston University, Boston MA Graduate Coursework in Bioinformatics University of Pennsylvania, Philadelphia, PA Graduate Coursework and original research in Neurochemistry Lebanon Valley College, Annville, PA Bachelor of Science ­ Biology PEER REVIEWED PUBLICATIONS: : Hadjiargyrou M, Lombardo F, Zhao S, Ahrens W, Joo J, Ahn H, J. M, WhiteDW, Rubin CT. Transcriptional profiling of bone regeneration. Insight into themolecular complexity of wound repair. J Biol Chem. Aug ;:. Epub Jun . : Holmqvist MH, Cao J, HernandezPineda R, Jacobson MD, Carroll KI, Sung MA,Betty M, Ge P, Gilbride KJ, Brown ME, J. ME, Lawson D, SilosSantiago I, Xie Y, Covarrubias M, Rhodes KJ, Distefano PS, An WF. Elimination of fastinactivation in Kv Atype potassium channels by an auxiliary subunit domain.Proc Natl Acad Sci U S A. Jan ;:. : Manji GA, Wang L, Geddes BJ, Brown M, Merriam S, AlGarawi A, Mak S, Lora JM, Briskin M, J. M, Cao J, DiStefano PS, Bertin J. PYPAF, a PYRINcontainingApaflike protein that assembles with ASC and regulates activation of NFkappaB. J Biol Chem. Mar ;:. Epub Jan . : Holmqvist MH, Cao J, Knoppers MH, J. ME, Distefano PS, Rhodes KJ, Xie Y,An WF. Kinetic modulation of Kvmediated Acurrent by arachidonic acid isdependent on potassium channel interacting proteins. J Neurosci. Jun;:. : Geddes BJ, Wang L, Huang WJ, Lavellee M, Manji GA, Brown M, J. M, Cao J,Morgenstern J, Merriam S, Glucksmann MA, DiStefano PS, Bertin J. Human CARD is a novel CED/Apaf family member that induces apoptosis. Biochem Biophys ResCommun. Jun ;:. : Weiger TM, Holmqvist MH, Levitan IB, Clark FT, Sprague S, Huang WJ, Ge P, WangC, Lawson D, J. ME, Glucksmann MA, SilosSantiago I, DiStefano PS, Curtis R. A novel nervous system beta subunit that downregulates human large conductancecalciumdependent potassium channels. J Neurosci. May ;:. : Liu Y, Holmgren M, J. ME, Yellen G. Gated access to the pore of avoltagedependent K+ channel. Neuron. Jul;:. : Holmgren M, J. ME, Yellen G. Ntype inactivation and the SS region ofthe Shaker K+ channel. J Gen Physiol. Sep;:. PubMed PMID:; : Liu Y, J. ME, Yellen G. Dynamic rearrangement of the outer mouth of a K+channel during gating. Neuron. Apr;:. : J. ME, Boland LM, Liu Y, Yellen G. Visual identification of individualtransfected cells for electrophysiology using antibodycoated beads.Biotechniques. Nov;:. . : Yellen G, Sodickson D, Chen TY, J. ME. An engineered cysteine in theexternal mouth of a K+ channel allows inactivation to be modulated by metalbinding. Biophys J. Apr;:. : Boland LM, J. ME, Yellen G. Cysteines in the Shaker K+ channel are notessential for channel activity or zinc modulation. Biophys J. Mar; Pt:. : Kienker P, Tomaselli G, J. M, Yellen G. Conductance mutations of thenicotinic acetylcholine receptor do not act by a simple electrostatic mechanism. Biophys J. Feb; Pt :. : Tomaselli GF, McLaughlin JT, J. ME, Hawrot E, Yellen G. Mutationsaffecting agonist sensitivity of the nicotinic acetylcholine receptor. Biophys J. Sep;:. : Yellen G, J. ME, Abramson T, MacKinnon R. Mutations affecting internalTEA blockade identify the probable poreforming region of a K+ channel. Science. Feb ;:. : Ohnishi ST, Horiuchi KY, Horiuchi K, J. ME, Sadanaga KK. Nitrendipine,nifedipine and verapamil inhibit the in vitro formation of irreversibly sickledcells. Pharmacology. ;:. : Krieger NR, Scott RG, J. ME. Testosterone alphareductase in rat brain.J Neurochem. May;:. : J. ME, Erulkar SD, Krieger NR. Testosterone alphareductase in spinalcord of Xenopus laevis. J Neurochem. Mar;:. : Erulkar SD, Kelley DB, J. ME, Zemlan FP, Schneider GT, Krieger NR.Modulation of the neural control of the clasp reflex in male Xenopus laevis byandrogens: a multidisciplinary study. Proc Natl Acad Sci U S A. Sep;:.

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