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Shingle Creek Crt.
Windsor, ON NW T
I am actively seeking a position as a
scientist in either a R&D or QC department of biotech/pharmaceutical
company or biologicalchemical diagnostic laboratory. My interdisciplinary
expertise also makes me qualified for a position of technical consultant/advisor
in the field of broad Life Sciences.
Experience and credentials are a hybrid
of advanced scientific knowledge and professional acumen based on interdisciplinary
background in Chemistry, Chemical Engineering and Life Science, including
M.Sc. in Chemistry and Chemical Engineering;
M.Sc. in Biochemistry. Incomplete MD degree.
+ years of experience in most
modern analytical techniques employed in research laboratory with either
chemical or biological profile as well as in methods development. DNA
& protein microarray technology and bioinformatics.
Expertise in the design, construction
and usage of phage display technology used for serum based biomarkers
for various tumors. Data mining and bioinformatics. Solid mathematical
skills. Working knowledge of GLP/GMP.
Multilanguage proficiency. Independent,
wellread, knowledgeable and crossdisciplinary trained professional.
aCGH expertise NimbleGene and
Superb interpersonal skills.
Indepth technical expertise on operation and troubleshooting of sophisticated
Detroit Medical Center, Cytogenetics
Laboratory, Detroit, MI March April
Chromosomal Microarray Technologist
Responsible for assay setup for the
NimbleGen CGX arrays, bioinformatics Genoglyphyx
Agilent SNP arrays.
DNA extraction and fluorescent probe
FISH probes labeling and purification.
Methods validation, SOPs preparation.
Karmanos Cancer Institute,
Wayne State University, Detroit, MI February Nov.
Responsible for assay setup,
development and implementing DNA and protein microarray technology cDNA
Development of phage display
technology in context of search for serumbased tumor biomarkers for
early cancer detection. Libraries design, construction several high
quality cellline and tumorbased libraries have been synthesized
Protein chips production spotting
and assay setup.
Maintenance, operating and troubleshooting
of chip printer as well as other peripheral devices, such as fluorescent
scanner, BIOMEK, etc.
Data mining & analysis, bioinformatics.
Literature search. Preparation
of scientific presentations and manuscripts.
Supervising and training graduate
students and visiting postdocs.
IntelliGene Expressions, Inc.,
Responsible for protein purification
and analysis of final products HPLC, Western blot, ELISA, IEF
Department of Cell Biology and Anatomy,
University of Alberta, Edmonton, AB
Research Assistant/Graduate Research
Molecular biology of yeast peroxisome
Department of Biochemistry,
University of Western Ontario, London, ON
Research Assistant/Graduate Research
Member of research group working on cholesterol
metabolism in rats.
Responsible for methods development and
chemical/biochemical analysis using gas chromatography, HPLC, column
chromatography, thinlayer chromatography, UV, IR, VIS spectroscopy,
analytical techniques employing isotopic tracers. Preparation of scientific
reports & presentations, library search.
GC, HPLC, TLC, column chromatography,
UV/IR/VIS/AA spectroscopy, classical
methods, including wet chemistry, electrochemical
methods, isotopic measurements, Karl
Fischer titration, dissolution methods, nephelometer;
Methods development & validation.
Biochemistry & Molecular Biology:
DNA/ RNA extraction & analysis, restriction
mapping, hybridization, Southern & Northern blotting, cloning techniques
E.coli, yeast, sequencing, PCR, RTPCR.
Protein extraction and purification,
Western blotting, ELISA, IEF, enzymology.
Microbiology & aseptic techniques.
Experience in animal care rats &
mice, small animal surgery, tissue cell cultures.
Processing and handling serum and other
DNA and protein microarray technology;
phage display technology:
Expertise in the design, construction
and usage of phage display libraries. Phage based and protein chips
printing, analysis; assay setup, cloning techniques in various systems
bacteria, yeast ;
High throughput DNA and protein sequence
cDNA chip and Affymetrix, NimbleGen CGX
Clustering and data mining tools: SpotfirePro,
GeneSpring, EPICLUST, BioDiscovery, Partek, Genoglyphix; Webbased molecular
biology tools and data bases, etc..
Statistical management of experimental
data using data analysis programs Sigma Plot, SStat, MatLab, etc.;
statistical tools employed in microarray technology. Solid mathematical
Biological data visualization, biological
network analysis. Knowledge of major public biological databases.
Advanced computer literacy; familiarity
with most utility software; data mining, Bioconductor/R; programming
languages: R, Perl.
Computer platforms: Windows, Windows
Working knowledge of GLP/GMP.
Proficiency in writing of technical reports
and making presentations.
Teaching experience: tutoring and TA
in undergraduate course in Biochemistry University of Western Ontario.
Languages written and spoken: Polish,
Russian, Dutch, German
Management, programming and operation
of sophisticated laboratory equipment BIOMEK, microarray printer, fluorescent
Oxysterols as regulators of cholesterol
Several components of naturally oxidized cholesterols were identified
as potent regulators of cholesterol absorption and cholesterol metabolism.
They were subsequently patented by the University of Western Ontario
ERregulated gene expression in
MCFAT and MCF cell lines.
A novel group of genes which were
potentially regulated by estrogen receptor and inhibited by antiestrogens
tamoxifen has been identified by cDNA microarray studies. The results
were then confirmed by experiments using Affymetrix chip.
Breast cancer biomarkers
A phagedisplay based approach has
been employed to screen potential immunereactive predictive biomakers
for early stage breast cancer. Several potential biomarkers have been
The project involved preparation
of specific phage libraries, biopanning against cancer patient sera,
printing of phagearrays, immunological assay and complex statistical
Significant contribution to the patent
issued in the name of the University
of Western Ontario in .
Contribution to the patent serumbased
biomarkers for breast cancer issued for the Wayne State University
Development of new original methods
of separation and purification of oxysterols TLC, column chromatography.
University of Western Ontario,
“Evidence of inhibition of
cholesterol absorption in rats by naturallyoccurring oxysterols”
Academy of Medicine currently School
of Medicine, University of Wroclaw, Wroclaw, Poland
Medical School ;
MD unfinished, completed /
of medical degree.
Technical University of Silesia,
M.Sc. Eng. Chemistry and Chemical
Engineering; Technology of Polymers
Book Chapter: Chatterjee,
M., W., J., Tainsky, M.A., Discovery of antibody biomarkers
using protein microarrays of tumor antigens cloned in high throughput,
chapter in “Tumor Biomarker Discovery”, Tainsky, M.A. Editor,
Pauley, RJ, Santner, SJ, W.,
J., Tainsky, MA, Tamoxifen and estrogen effects on gene expression in
prenoplastic MCFAT and neoplastic MCF cells, abstract, th Annual
Meeting AACR, , Toronto, ON, Canada
Lin, H.S., et al. , Autoantibody
approach for serumbased detection of head and neck cancer, Cancer
Epidemiol Biomarker Prev., , , ;
W., J., et al, Proteomic
approaches to early detection of breast cancer, poster presentation,
Annual EDRN Meeting, Washington, DC, .
Dudas, S., W., J.,
Onchip diagnostic immunoassays of panels of bioselected tumor antigens:
binding to serum autoantibodies for the early detection of cancer, poster
presentation, Annual EDRN Meeting, Washington, DC,